Device

Part:BBa_K112808:Design

Designed by: Jin Huh   Group: iGEM08_UC_Berkeley   (2008-10-31)


Enterobacteria phage T4 Lysis Device - no promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1350
    Illegal NheI site found at 1373
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 955
    Illegal AgeI site found at 1025
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1606


Design Notes

[Pcon].[Antiholin].[terminator] was assembled first, and then assembled with [T4 holin].[terminator]; Constitutive expression of antiholin is required before assembling holin.


Source

Pubmed Accession Number: NC_000866 Enterobacteria phage T4

References